Microplates: August 2019

Tuesday, August 20, 2019

Porvair Sciences Manufacture a Wide Range of Microplate Products

Porvair Sciences manufacture a wide range of microplate products, as well as specialise, as well as serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, for example, sample preparation through their global distributor network. They also manufacture microplate products such as the 96 well plate.

The deep well microplates are commonly used for sample storage in life science laboratories. There is an important aspect associated with the manufacture of these plates, which is the selection of clean raw materials for injection moulding.

They are made of polypropylene, which is an inert and heat resistant material, ideal for sample storage. However, all grades of polypropylene are not the same; for example, injection moulding grades of polypropylene often have high concentrations of chemicals to aid the moulding process. This allows rapid production of plates and lower costs, but may compromise the integrity of samples or compounds which are stored in such plates for extended periods.

With regards to one of the microplates, the 96 deep well plate was designed so that it would make the interchange of plates simpler in automated systems. So, for applications using the GenoGrinder™ and TissueLyzer™, a specially reinforced version of this plate is available with extractable-free, medical-grade molecules called polymer.

The microplate 96 well is specifically for seed and leaf genomic studies, which is an area within genetics that focuses on the analysis of an organism. The microplate 96 well is also:

Made from virgin polypropylene
Tested for low extractables
Tight specification levels
It can allow total liquid removal
There are no inner edges
Manufactured under DNase/ RNase free environment
Working volumes of 350µl, 1ml, 1.6ml and 2ml
Raised well rims to improve heat sealing
It has a conical base
Sterile and non-sterile versions

These microplates are made in virgin polypropylene to minimise extractable. Their maximum volume is 2.075 ml per well, and working volume is a useful 1.85 ml, more than any other comparable ‘2 ml Round Well’ plate in this class.

There is a design called the ‘common wall’ which allows the highest possible volume to be used while maintaining an overall height of just 45 mm, complete with ANSI/SLAS standard base and footprint.

Fortunately, Porvair Sciences has developed several storage/collection plates to help in the fields of cell biology, molecular biology, drug discovery, combinatorial chemistry, screening and genomics (an area within genetics).

The plates are manufactured in proper room conditions, and a significant number are DNase/RNase free. They are mainly made from polypropylene, which is inert material giving heat and solvent resistant qualities.

At Porvair Sciences, there is a new range of low profile 1.1ml 96-well round plates. Thus, more plates can be stored or stacked in a given space. As mentioned previously, since the plates are made with extractable-free polypropylene, this, in turn, gives excellent results. Also, there is a specific anti-evaporation cap mat available.

Porvair Sciences offers the most significant combination of excellent shape, several wells and well volume on the market. Each one of the good microplates is in the ANSI/SBS format, this is found on most washers and mechanical equipment.

Contact the Microplates team today if you require more information or if you have any questions or queries. You can call 02920 480720 or email us on cbrownhill@spindogs.com

SOURCE:https://thenewsfront.com/porvair-sciences-manufacture-a-wide-range-of-microplate-products/.

Company Name: Microplates
Contact Person: Chelsea Brownhill
Email: Send Email
Phone: +44 (0)1978 661144
Address:Clywedog Road South Wrexham Industrial Estate
City: Wrexham LL13 9XS
Country: United Kingdom
Website: https://www.microplates.com

Porvair Sciences Offers a Complete Range of Products to Implement SPE

Porvair Sciences offers a wide range of products to implement SPE extraction. Solid-phase extraction (SPE) is a method of sample preparation that concentrates and purifies analytes from solution by sorption, followed by elution of the analyte with a solvent appropriate for instrumental analysis, such as LC-MS.

MicroLute™ is the original SPE 96-well plate format for SPE. According to Porvair Sciences, the device consists of a ‘polypropylene 96-well plate loaded with a choice of sorbent and sorbent volumes’. Also, they mention that the frits have a ‘mean pore size of 20 microns and are made from microporous high-density polyethylene’.

Solid-phase extraction uses the difference of attraction between an analyte and interferents, present in a liquid matrix, for a solid phase (sorbent). It is the attraction that allows the separation of the target analyte from the interferents.

There are several steps involved with a solid-phase extraction; they include:

First, the cartridge is conditioned with a solvent to wet the sorbent. After that, the loading solution containing the analyte is infiltrated through the solid phase (SP). Ideally, the analyte and some impurities are retained on the sorbent. The sorbent is then washed to remove impurities, and the analyte is collected during this elution step.

Porvair Sciences have a vast range of products which includes; vacuum manifolds, sealers, evaporators and microfilter plates, deep well and shallow good storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs.

Porvair Sciences also provides custom microplate products for life science research. Their vacuum manifolds are essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

Porvair Sciences mention in their words that solid-phase extraction (SPE) is a sample preparation technique generally used in analytical laboratories for the removal of analytes from a complex matrix. This sample preparation technique helps with the extraction, the clean-up and the concentration of analytes before their quantification.

Additionally, solid-phase extraction can take less the chances of problems occurring, with liquid-liquid extraction, and this, in turn, improves quantitative recovery yields. It is a quick technique (most extraction in less than 30 min), easy to perform and can be automated. Also, this technique is suitable for the pre-treatment of complex matrices such as urine, blood, food samples, water etc.

A broad range of phases exists for SPE. Most of them are based on ionic, hydrophobic or polar interactions. Their selectivity is limited, and the target analyte often co-elutes with interfering compounds. On the other hand, there are molecularly imprinted polymers are the most highly selective phases available today. This technology enables to reduce the presence of interferents during the elution step and obtain higher recoveries in chromatography analysis even at low levels.

Porvair Sciences are experts in the business, and of the development of molecularly imprinted polymers, they have developed a range of selective solid Phase Extraction sorbents using the molecular imprinting procedure for sample clean-up before analysis.

It is evident that solid-phase extraction is a well-established technique, and fortunately, Porvair Sciences offers a wide range of products to implement the SPE extraction.

Contact Microplates today if you require more information about SPE extraction, SPE plates or SPE frits. If you have any questions or queries, you can call +44 (0)1978 661144 or email cbrownhill@spindogs.com

SOURCE:https://thenewsfront.com/porvair-sciences-offers-a-complete-range-of-products-to-implement-spe/.